ABSTRACT
Spirulina sp., multicellular filament algae, is helically
coiled. This is a rich nutrition microalgae with protein,
carbohydrate, vitamin, chlorophyll and carotenoid. Many
researches and applications of Spirulina sp. have been studied by
interested scientist, especially, pigment production. The purpose
of this research was determination the effect of pH, dark – light
cycle and light colour on the growth and pigment production of
Spirulina sp. The results showed that pH = 9, white light and
continuous illumination 24/24 hours were appropriate conditions
for biomass increasing, chlorophyll a, chlorophyll b and
carotenoid production in Spirulina sp.
At pH = 9, biomass and carotenoid were highest in day 8
(0.16 g/ 50 mL and 1.43 µg/mL, respectively), the highest
production of chlorophyll a and chlorophyll b were collected in
day 12 (2.72 µg/mL and 3.35 µg/mL). The growth of Spirulina sp.
was slow at green colour and limited at red colour.
Compared to 12/24 hour illumination, growing algae under
continuous illumination 24/24 hour was higher 1.08 times in
biomass, 2.36 times in chlorophyll a, 1.2 times in chlorophyll b
and 1.7 times in carotenoid. When white light was applied,
continuous illumination 24/24 hour and aeration, pH remained
from 10 to 10.8, decreased in day 16 and 20 and no significant
differences between treatments during the experiment. Besides,
Spirulina algae recovered quickly in appropriate conditions.
Key words: biomass, carotenoid, chlorophyll a, chlorophyll
b, Spirulina sp.
CONTENTS
APPROVAL
ABSTRACT ............................................................................... i
CONTENTS ............................................................................... ii
1. INTRODUCTION .................................................................. 1
1.1 Introduction....................................................................... 1
1.2 Objectives ........................................................................ 2
2. MATERIALS AND METHODS ........................................... 3
2.1 Materials ........................................................................... 3
2.1.1 Time and location ...................................................... 3
2.1.2 Tools and equipments ................................................ 3
2.2 Methods ............................................................................ 3
2.2.1 Microalgae biomass increasing, chlorophyll and
carotenoid extraction method ............................................. 3
2.2.2 Study the effect of pH on Spirulina sp. chlorophyll
and carotenoid production .................................................. 5
2.2.3 Study the effect of light colour on Spirulina sp.
chlorophyll and carotenoid production................................ 5
2.2.4 Study the effect of dark – light cycle on Spirulina sp.
chlorophyll and carotenoid production ............................... 6
2.2.5 Study the growth recovering of Spirulina sp. ............. 6
2.2.6 Data analysis method ................................................. 6
3. RESULTS AND DISCUSSIONS ........................................... 7
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